Characterization of thermophilic microflora and thermostable biocatalysts for commercial applications
Thermostable enzymes isolated form thermophilic bacteria are lately realized to be advantageous over chemical industrial catalysts because they offer robust catalytic alternative, rarely require toxic metal ions for functionality, able to withstand harsh conditions of industrial processing, operational stability at high temperature and denaturant tolerant. In the face of soaring energy costs, dwindling fossil resources, environmental pollution and a globalized economy, the large scale use of biotechnology instead of, or to complement, traditional industrial production processes, particularly in chemical sector, is viewed both as an opportunity and a necessity. Our group focuses on the utilization of novel biotechnological application for possible genetic analysis for total genome of thermophilic bacteria, characterization of noble strains for industrially important thermostable enzymes and process optimization from industrial prospective following in vitro biochemical assay and/or metagenomic analysis for large-scale production of selected thermostable biocatalysts.

Teaching Experience (Biotechnology): 10 years

Post	Organization	Duration
Assistant Professor	Center of Biotechnology, SOA University, Bhubaneswar	since 2009
Senior Lecturer	Center of Biotechnology, SOA University, Bhubaneswar	2006-2009
Lecturer	Amity Institute of Biotechnology, Bhubaneswar,	2004-2006
Visiting  Lecturer	Ravenshaw Unitary University, Cuttack Odisha	2003-2004
Lecturer	Career Point, Bhanjanagar, Odisha	1998-2001

Industry Experience (Biotechnology): 5 years

Product Development Officer:   Name of organization          Duration	Microbial Biofertilizer and Biopesticide product development, routine Lab-land-lab analysis and promotion. International Panacea Ltd., New Delhi 2001 –2004
Scientist, Quality Control:        Name of organization             Duration	Biotechnological evaluation of food and dehydrated vegetable samples. Jain Foods, Jain groups of company, Jalgaon, Maharastra.  1996 -1998

Research experience (Biotechnology): 11 years

Current Research: Place of Work Duration	Biotechnological intervention for searching new molecules with antimicrobial potential of Plant origin Characterization micro flora of Thermal ponds Siksha O Anusandhan University, Bhubaneswar Since 2006
Research work for Ph.D in (Biotechnology): Name of organization Duration Place of work	Molecular characterization of ginger (Zingiber officinale) and in vitro conservation of some promising cultivars. Department of Biotechnology, Utkal University, Bhubaneswar 2006- 2010 Siksha O Anusandhan University, Bhubaneswar
Research Project: Funding Agency Duration Place of work	Evaluation of potential of microbial biomass to sequester metal ions from industrial wastewater. Amity University, New Delhi September 2004 - March 2006 Amity Institute of Biotechnology, Bhubaneswar
Junior Research Fellow  (Ministry of Environment & Forest, New Delhi): Name of organization Duration	Case study of analysis of metallurgical industrial effluents and Biotechnological solution for waste water treatment of metal producing industries like H.C.L, Ghatsila & H.Z.L,  Vizag Dept. of Biotechnology & Biominerology Regional Research Lab, (CSIR), Bhubaneswar May 1994 ¾ Dec 1996
Research work for M.Tech: Name of organization Duration	Potential of Rhizopus arrhizus biomass for removal of metals from metallurgical effluents. IIT, Kharagpur, Applied Botany Division, Agriculture & Food Engg. Department, July 1992 – May 1994

• Presented paper at International Biotechnology Symposium, Dalian City, China IBS-2008.

Total number of publications: 33
Papers published in International journals: 15 (Published), 3 (Accepted)
Book Chapter published: 2
Papers presented in Seminar/conference: International-4 and National-9

• R. K. Sahoo, A. Kuanar, R. K. Joshi, A. P. Das, S. Nayak, E. Subudhi (2011) Antidermatophytic activity of eucalyptol rich turmeric somaclone oil against human pathogenic isolates. Journal of medicinal plant research Vol. 5(9), pp. 1594-1597, (I.F:0.59, Indexed in SCOPUS)
• RK Joshi , B Kar , S Mohanty , E Subudhi , S Nayak  (2011), Molecular Cloning, Characterization, and Expression Analysis of Resistance Gene Candidates in Kaempferia galanga L. Mol Biotechnol.   DOI:  10.1007/s12033 - 011-9430 - 6,v    (I.F 2.7,indexed in SCOPUS)
• S. Singh, B.S.Satapathy, R.K.Sahoo, E.Subudhi and S. Nayak (2011), In vitro validation and phytoconstituent analysis of turmeric extract: An ethnological alternative for eye treatment, Research journal of medicinal plants. 5(3): 330-337 indexed in SCOPUS
• E. Subudhi, S Mohanty, S Mohanty, A Kuanar, M K Panda (2010)."In Vitro Antimicrobial Study of Plant Essential Oils and Extracts". Internet journal of Microbiology. 8(1). Indexed in SCOPUS
• E. Subudhi, S Mohanty, R K Joshi, R C Mohanty, S.Nayak. (2010)  Retention of drug yielding potential of long term micropopagated gingers through periodic monitoring of genetic stability in vitro. Journal of Pharmacy Research, 3(10), 2421-2424. (I.F 1.09, 2009, Indexed in SCOPUS)
• S Nayak, T Kaur, S Mohanty, G Ghosh, R Choudhury, L  Acharya, E Subudhi (2010) In-vitro and ex-vitro evaluation of long-term micropropagated turmeric as analyzed through cytophotometry, phytoconstituents, biochemical and molecular markers.  64(1), 91-98, Plant Growth regulations. ((I.F: 2.06, Indexed in SCOPUS)
• R. K. Joshi, S. Mohanty, E. Subudhi, S. Nayak (2010). Isolation and characterization of NBS-LRR resistance gene candidates in Curcuma longa cv. surama. Genetics and Molecular Research 9(3): 1796-1806. (I.F: 0.82, Indexed in SCOPUS)
• R.K.Joshi, , E. Subudhi B Kar and S Nayak (2010) Comparative genetic analysis of lowland rice cultivars of india using microsatellite markers Bioresearch Bulletin 4: 213-223 Indexed in SCOPUS
• S Singh, A Kuanar, S Mohanty, E. Subudhi and S Nayak (2010) Evaluation of phytomedicinal yield potential and molecular profiling of micropropagated and conventionally grown turmeric (Curcuma longa L.) (104)2, 263-269. Plant Cell Tissue and Organ Culture, (I.F: 1.2,  Indexed in SCOPUS)
• R.K.Joshi, A.Kuanar, S. Mohanty, E. Subudhi and S.Nayak (2010). Mining and characterization of EST derived microsatellites from Curcuma longa L. Bioinformation, 5(3) 128-131. (I.F: 0.87, Indexed in PubMed)
• S Mohanty, R Parida, S Singh, R K Joshi, E Subudhi, S Nayak (2010)   Biochemical and molecular profiling of micropropagated and conventionally grown Kaempferia galanga. (106)1, 39-46 Plant Cell Tissue Organ Culture. (I.F: 1.2, Indexed in SCOPUS)
• S Singh, M K Panda, E. Subudhi and S Nayak (2010). Chemical Composition of Leaf and Rhizome Oil of an Elite Genotype Curcuma longa L. from South Eastern Ghats of Orissa. Journal of Pharmacy Research, 3(7):1630-1633. (I.F 1.09, 2009, Indexed in SCOPUS)
• S Mohanty, R K Joshi, E. Subudhi, S Sahoo, S Nayak (2010). Assessment of genetic stability of micropropagated Curcuma caesia through cytophotometric and molecular analysis. Cytologia 75(1): 73–81. (I.F: 0.2, Indexed in SCOPUS)

• S. Singh, B.S.Satapathy, R.K.Sahoo, E.Subudhi and S. Nayak (2011), In vitro validation and phytoconstituent analysis of turmeric extract: An ethnological alternative for eye treatment, Research journal of medicinal plants. DOI: 10.3923 / rjmp. 2011 Indexed in SCOPUS
• E. Subudhi, S Mohanty, S Mohanty, A Kuanar, M K Panda (2010)."In Vitro Antimicrobial Study of Plant Essential Oils and Extracts". Internet journal of Microbiology. 8(1). Indexed in SCOPUS
• E. Subudhi, S Mohanty, R K Joshi, R C Mohanty, S.Nayak. (2010) Retention of drug yielding potential of long term micropopagated gingers through periodic monitoring of genetic stability in vitro. Journal of Pharmacy Research, 3(10), 2421-2424. Indexed in SCOPUS
• R K Joshi, S Mohanty, E. Subudhi, S Nayak (2010). Isolation and characterization of NBS-LRR resistance gene candidates in Curcuma longa cv. surama. Genetics and Molecular Research 9(3): 1796-1806. Indexed in SCOPUS
• R.K.Joshi, A.Kuanar, S. Mohanty, E. Subudhi and S.Nayak (2010). In-silico mining and characterization of EST derived microsatellites from Curcuma longa L. Bioinformation, 5(3) 128-131. Indexed in PubMed
• S Singh, A Kuanar, S Mohanty, E. Subudhi and S Nayak (2010) Evaluation of phytomedicinal yield potential and molecular profiling of micropropagated and conventionally grown turmeric (Curcuma longa L.). Plant Cell Tissue and Organ Culture, DOI: 10.1007/s11240-010-9811-8. Indexed in SCOPUS
• S Singh, M K Panda, E. Subudhi and S Nayak (2010). Chemical Composition of Leaf and Rhizome Oil of an Elite Genotype Curcuma longa L. from South Eastern Ghats of Orissa. Journal of Pharmacy Research, 3(7):1630-1633. Indexed in SCOPUS
• S Mohanty, R K Joshi, E. Subudhi, S Sahoo, S Nayak (2010). Assessment of genetic stability of micropropagated Curcuma caesia through cytophotometric and molecular analysis. Cytologia 75(1): 73–81. Indexed in SCOPUS
• S. Kumar, E. Subudhi, S. Nayak and S. Sahoo (2009) Antimicrobial activity of essential oils from 15 lemongrass genotypes - a comparative assay. Internet journal of Microbiology. 6 (1). Indexed in SCOPUS
• E.Subudhi and R.N.Kar  (2008) Rhizopus arrhizus – An efficient fungus for copper effluent treatment. International journal of integrative Biology. 2 (3) 166-171. Indexed in SCOPUS
• S.Mohanty, M.K.Panda, E.Subudhi, L.K.Acharya and S.Nayak (2008) Plant regeration from callus culture of Curcuma aromatica Salisb. and in vitro detection of somaclonal variation through cytophotometric analysis  Biologia Plantarum 52(4), 783-786. Indexed in SCOPUS
• S.Mohanty, M.K.Panda, E.Subudhi and S.Nayak (2008) Genetic stability of micropopagated ginger derived from axillary bud through cytophotometric and RAPD analysis. Z. Naturforsch. 63c: 747-754. Indexed in SCOPUS
• S. Ghosh, E. Subudhi and S. Nayak (2008) Antimicrobial assay of Stevia rebaudiana Bertoni leaf extracts against 10 pathogens. International journal of integrative Biology 2 (1) 27-31.  Indexed in SCOPUS
• M.K.Panda, S.Mohanty and E. Subudhi and S. Nayak (2007) Assessment of genetic stability of micropropagated plants of Curcuma longa L. by cytophotometry and RAPD analyses. International journal of integrative Biology. 1:189-195. Indexed in SCOPUS
• E.Subudhi and R.N.Kar (1996) Decontamination of metals from metallurgical effluents utilizing R. arrhizus biomass, International journal of environmental studies 51 (20): 111-116. Indexed in SCOPUS

• S Mohanty, R Parida, S Singh, R K Joshi, E Subudhi, S Nayak (2010) Biochemical and molecular profiling of micropropagated and conventionally grown Kaempferia galanga. Plant Cell Tissue Organ Culture (In Press). Indexed in SCOPUS.
• S Nayak ,T Kaur , S Mohanty, G Ghosh, R Choudhury, L  Acharya, E. Subudhi (2010) In-vitro and ex-vitro evaluation of long-term micropropagated turmeric as analyzed through cytophotometry, phytoconstituents, biochemical and molecular markers. Plant Growth regulations (In Press) Indexed in SCOPUS.
• R. K. Sahoo, A. Kuanar, R. K. Joshi, A. P. Das, S. Nayak, E. Subudhi Antidermatophytic activity of eucalyptol rich turmeric somaclone oil against human pathogenic isolates. Journal medicinal plant research (In Press) Indexed in SCOPUS

• S.P.Mishra, E.Subudhi, P.K.Biosoi, G, Ray Choudhury, R.N.Kar, L.B.Sukla (1995) “Biosorption of copper from copper industrial effluents” a case study, Regional research larboratory, Bhubaneswar. Edited by Sastri, S.R.S. et al, Allied Publishers Ltd., New Delhi, pp 385-390.
• E.Subudhi and R.N.Kar. (2007) “Biosorption of Rhizopus arrhizus biomass to accumulate Zinc from its Aqueous Solution”. MB-2007, National Seminar on Mineral Biotechnology at R.R.L, Bhubaneswar. Jan 30-31, Published in edited book, “Mineral Biotechnology” pp 147-155.

R K Joshi, S Mohanty, E Subudhi, S Nayak. Isolation and characterization of Curcuma NBS analogs. GenBank HM117001 - HM117005.

International

• R.K.Sahoo, R. Parida and E.Subudhi (2010) “Isolation and characterization of bacteria from thermal pond of orissa, India” International symposium and 51th annual conference organized by AMI of India at BIT, Mesra, Ranchi.14-17th Dec.
• E.Subudhi, S.Mohanty, M.Panda, L.K.Acharya and S.Nayak (2008): Genetic stability of micropropagated ginger cultivars as assessed through in vitro and ex vitro evaluation. IBS2008,Dalian, China 12-17th Oct 2008.
• S.Nayak, S.Mohanty and E.Subudhi (2008) Differential synthesis of essential oil in callus derived microshoots of turmeric (Curcuma longa L.) in vitro. IBS2008, Dalian, China 12-17th Oct 2008.
• E.Subudhi and R.N.Kar (1994) “Bioaccumulation of dead R. arrhizus” in international conference MICON International-94 and 34th Annual conference of AMI at CDRL, Mysore, 9-12th Nov.
  
  National:
• E.Subudhi (2009) “Industrial fungal waste and safe environment” in Proceedings of the national seminar on-Industrially important microbes and their biosafety on 27th March, Utkal University, pp-48-57.
• E.Subudhi (2008) “Differential anti microbial activity of essential oil of 15 promising lemongrass cultivars”, National seminar on 31st March Utkal University, Bhubaneswar.
• S.Mohanty, M.K.Panda, E.Subudhi and S.Nayak (2008) “Micropropagation of Zingiber officinale  Rose. using axillary buds of rhizome and in vitro assessment of genetic stability using cytophotometry and RAPD analysis”. 29th National symposium of plant tissue culture association. Udayapur, Rajasthan (India) pp-8.
• E.Subudhi (2007) “Pharmcogenomics”, In proceedings of AICTE sponsored national seminal on “Drug Design & New Drug Discovery Techniques” at School of pharmaceutical sciences, ITER, Bhubaneswar.
• E.Subudhi (2007) “Application liquid biofertiliser in crop plants”, National seminal on 28th March at Utkal University, Bhubaneswar.
• E.Subudhi and R.N.Kar. (2006) “Utilisation of Rhizopus arrhizus biomass to sequester zinc from its aqueous solution.” National Seminar and 47th Annual Conference of Association of Microbiologists of India, Microbiology-the challenges ahead, Bhopal, 6-8 Dec.
• E.Subudhi (2005) “Quality Biofertilizer in Organic Agribusiness”, in Promotion and management of agri-business enterprises, sponsored by UGC, New Delhi, organized by Utkal University, Bhubaneswar, 20th March.
• E.Subudhi and R.N.Kar. (1994) “Microbial accumulation of zinc from acqueous solution using R. arrhizus biomass” at 34th Annual conference of AMI, at PAu, Ludhiana, 9-11th Feb.
• 13. E.Subudhi and R.N.Kar. (1993) “Biosorption of copper by Rizopus arrhizus” in national conference Hydrotech-93, at RRL, Bhubaneswar, 5-6th Oct, pp 180-184.