Profile of Mr. Raj Kumar Joshi ( Assistant Professor )::

Mr. Raj Kumar Joshi
Department: Biotechnology
Designation: Assistant Professor
E-mail ID: rkjoshi@spsiter.ac.in
Contact Number: +919437684176
Date of Birth: 20 th July, 1982
Date of joining: 1 st July, 2009
Status as on date of joining: Lecturer
Present status: Assistant Professor
Educational Qualifications
Sl. No. Degree University Year Subjects Percentage
1 B.Sc. Sambalpur University 2002 Botany Honors 76.4%
2 M.Sc. Sambalpur University 2004 Biotechnology CGPA= 7.9/10
3 Ph.D. Siksha O Anusandhan University 2011 Biotechnology  
Experience In Research Techniques
  • Molecular cloning, detection, characterization and expression analysis of resistance genes in turmeric and Kaempferia.
  • DNA and RNA isolation, plasmid purification, polymerase chain reaction, RT-PCR analysis and Nucleotide Binding Site based motif directed profiling.
  • RAPD, ISSR, AFLP and SSR analysis of important medicinal plants.
  • In silico detection, development and characterization of SSR markers in medicinal plants.
  • Utilization of various bioinformatic tools for sequence characterization, alignment, phylogenetic analysis and conserved motif validation of various genes and proteins.
  • Marker based characterization of aromatic rice accessions.
  • Marker assisted gene pyramiding for developing biotic stress resistance in crop plants.
Work Experience
Position Organization Duration
Lecturer Royal School of management and Technology, Bhubaneswar September 2004- August 2005
Senior Research Fellow Central Rice Research Institute, Cuttack November 2005-May 2009
Assistant Professor Siksha ‘O’ Anusandhan University July 2009-till date
AREA OF RESEARCH
Molecular cloning and characterization of genes and promoter for plant stress tolerance and signaling transduction in asexually reproducing plants. Plants are exposed to a variety of biotic and abiotic stresses. To survive under these challenges, plants have developed elaborate signal transduction mechanisms to counter act against the external signals and establish cellular responses. Plant pathogen interaction is high complex due to their differential recognizing abilities and varied resistant mechanism. Turmeric is a plant of great medicinal and economic significance. However, its productivity is not upto the demand owing to various biotic and abiotic stress incidences. Therefore, we are trying to clone and characterize the genes controlling signaling modules as well as plant R-genes to get better insights about the organization, function and modulation of defense response and performances underlying signal transduction pathway of asexually reproducing plant attacked by pathogens.
Dissertation Completed
  • Ph.D. Thesis Title:  “Molecular cloning and characterization of disease resistance gene candidates in Curcuma longa and Kaempferia galanga with special reference to NBS profiling in Zingiberaceae”.
  • M.Sc. Thesis Title: “Identification and differentiation of indeginous non Basmati aromatic rice genotypes of India using microsatellite markers''.
Awards
  • 2005  Received the Senior Research Fellowship from Indian Council Agrucultural Research (ICAR) under the scheme “Gene pyramiding for resistance against biotic stress resistance in crops”.
  • 2004 Gold medal for securing first position in M.Sc. Biotechnology, Sambalpur University.
  • 2002 Gold medal for securing first position in B. Sc. Biotechnology, Sambalpur University.
Research Experience (6.5 Years)
I have six and half years of research experience in modern plant breeding techniques such as Marker-assisted selection, Gene pyramiding, double haploid breeding, tissue culture and transgenic development.
My present area of research focus on identification, cloning, characterization and expression studies of resistance and regulatory gene candidates in the family of Zingiberaceae.
Professional Overview
  • Lecturer: Working as Lecturer in molecular biology and genetic engineering at Centre of Biotechnology, Siksha O Anusandhan University, Bhubaneswar, INDIA. (July 2009 till date)
  • Senior Research Fellow: Worked as Senior Research Fellow under ICAR Net work project entitled “Gene pyramiding for developing resistance against bacterial leaf blight, blast and gall midge in rice funded by Indian Council of Agricultural Research (ICAR), Govt.of.India at Central Rice Research Institute, Cuttack, India. (October’2005 –June 2009).
  • Lecturer: Worked as lecturer in Cell & Molecular biology at Royal School of Management and Technology (RSMT) under Utkal University, Bhubaneswar, India. (September 2004-August 2005)
  •  Research Intern- Awarded with research internship at Masters Level to work on “DNA finger printing of aromatic rice” (supervisor- Dr. S.C.Sahu, at Central Rice Research Institute, India (funded by DBT, Govt.of.India) (March 2004-July 2004).
Technical Expertise
  • Plant cell culture techniques: In-vitro micro propagation in Zingiberaceae and anther culture of different indica rice
  • Alien Gene Transformation methods: Agrobacterium- and biolistic mediated
  • DNA methodologies: Extraction and estimation of concentration of total DNA by using fluorometry, DNA fingerprinting,Polymerase Chain Reaction (PCR), RT- PCR, Primer designing, Southern hybridization, Molecular cloning procedures for preparation of recombinant plasmids, molecular breeding, development of molecular markers, genome sequencing, restriction digestion etc.
  • Biochemical and electrophoretic techniques: Isolation of total RNA, purification extraction of total Protein and quantification by using Barfords assay, SDS-PAGE and agarose gel electrophoresis.
  • Immunochemical techniques: ELISA, and western blotting.
  • Bioassay of plants: against Rice disease and insect pest (Bacterial leaf blight, fungal blast and gall midge).
Instruments Handled
  • Particle gene gun (PDC-100 Biorad).
  • UV-Vis.spectrophotometer (Shimadzu).
  • Fluorometry (Hoefer TKO100 minifluorometer).
  • Multiple blocks - Programmable Thermal Cycler (PCR) -100 (Eppendorf & MJ    Research).
  • ELISA reader.
  • Gel documentation system (Alpha Innotech 2200).
  • Horizontal Electrophoresis systems.
  • Vertical gel electrophoresis and sequencing systems (BIOTECH).
  • Simple and Compound microscope.
  • Ultra centrifuge
Presentations in Conference proceedings
  • R K Joshi and G J N Rao (2010) Marker-assisted gene pyramiding for cumulative resistance against bacterial Blight, Blast and Gall Midge in Rice. Proceedings of International conference on Green Plant breeding technologies, 2-5th February 2010, University of Vienna, Austria.
  • S Mohanty, R K Joshi, R Parida, E Subudhi and S Nayak (2010). Proceedings of National symposium on plant cell tissue and organ culture: the present scenario, 3-5th March, Centre of advanced study, dept. of Botany, University of Kolkata.
  • S Mohanty, R Parida, B Kar, R K Joshi, E Subudhi and S Nayak (2010) Biochemical analysis of essential oil from micropropagated Kaempferia galanga. Proceedings of National conference on Sustainable production and utilization of medicinal and aromatic plants: Current trends and future prospects, 8-9th March 2010, Regional Plant Resource Centre, Bhubaneswar.
  • D.Prasad, R.K.Joshi, V.V.Pulla Rao and G.J.N.Rao (2006). Pyramiding of resistance genes for bacterial leaf blight through marker assisted selection in rice. 26th International Rice Research Conference. International Rice Congress, New Delhi,Pp274.
  • D.Prasad, R.K.Joshi, V.V.Pulla Rao and G.J.N.Rao (2006). Pyramiding of resistance genes for bacterial leaf blight through marker assisted selection in rice. National symposium on Molecular breeding in crop plants. Indian Institute of Vegetable Research, Varanasi. March20-21, Pp182.
Sequence submission to NCBI Database
  • R K Joshi, Mohanty S, Subudhi E and Nayak S (2010). Five NBS-LRR resistance gene analogs from Curcuma longa cv Surama, partial cds (Accession no. HM117001-HM117005).
  • R K Joshi, Kar B, Mohanty S, Subudhi E and Nayak S (2010). 14 NBS-LRR resistance like protein sequences from Kaempferia galanga (Accession no. HQ650147-HQ650160).
  • R K Joshi, Kar B, Mohanty S, Subudhi E and Nayak S (2011). 21 CC-NBS-LRR resistance gene candidates from three wild species of turmeric, Curcuma aromatica, Curcuma angustifolia and Curcuma zedoaria (Accession no. JN426969-JN426989).
List of Publications
PUBLICATIONS (Number only): 23
Research Papers- 18
Conference presentation-5
Papers communicated- 02
Patents: No
Research Paper Published
  • R K Joshi, Kar B, Mohanty S, Subudhi E and Nayak S (2011) Molecular cloning, characterization and expression analysis of resistance gene candidates in Kaempferia galanga L. Molecular Biotechnology. (DOI: 10.1007/s12033-011-9430-6). (IF: 2.6).
  • R K Joshi and Nayak S (2011) Functional characterization and signal transduction ability of the nucleotide binding site leucine rich repeats (NBS-LRR) resistance genes in plants. Genetics and Molecular Research.10 (4):2637-2652. (IF: 1.015).
  • R K Joshi, B Kar and S Nayak (2011) Characterization of mitogen activated protein kinases (MAPKs) in the Curcuma longa expressed sequence tag database. Bioinformation 7(4): 180-183. (IF: 1.15).
  • R K Joshi, B Kar, S Nayak (2011) Survey and characterization of NBS-LRR (R) genes in Curcuma longa transcriptome. Bioinformation 6(9): 360-363. (IF: 1.15).
  • S Mohanty, R Parida, S Singh, R K Joshi and S Nayak (2011) Biochemical and molecular profiling of micropropagated and conventionally grown Kaempferia galanga. Plant Cell Tissue and Organ Culture. 106 (1):39-46. (IF: 1.24).
  • R K Joshi, B Kar and Nayak S (2011) Exploiting EST databases for mining and characterization of short sequence repeat (SSR) markers in Catharanthus roseus L. Bioinformation. 5(9): 378-381. (IF: 1.15).
  • R K Joshi, S Mohanty, E Subudhi, S Nayak (2010) Isolation and characterization of NBS-LRR resistance gene candidates in Curcuma longa cv. surama. Genetics and Molecular Research9(3): 1796-1806. (IF: 1.015).
  • R K Sahoo, A Kuanar, R K Joshi, A P Das, S Nayak and E Subudhi (2011).  Anti-dermatophytic activity of eucalyptol rich turmeric somaclone oil against human pathogenic isolates Journal of Medicinal Plants Research 5(9):1594-1597. (IF: 0.876).
  • R K Joshi (2011) Development of an efficient marker assisted backcrossing scheme in rice using microsatellite markers. Research Journal of Biotechnology. 6(4): 19-26. (IF: 0.248).
  • R K Joshi, A Kuanar, S Mohanty, E Subudhi and S Nayak (2010) In-silico mining and characterization of EST derived microsatellites from Curcuma longa L. Bioinformation5(3): 128-131. (IF: 1.15).
  • R K Joshi and S Nayak (2010) Gene pyramiding- A broad-spectrum technique for developing durable stress resistance in crops. Biotechnology and Molecular Biology Reviews 5(3): 51-60. (IF: 0.458).
  • S Mohanty, R K Joshi, E Subudhi, S Sahoo, S Nayak (2010). Assessment of genetic stability of micropropagated Curcuma caesia through cytophotometric and molecular analysis. Cytologia 75(1): 73–81. (IF: 0.3).
  • E Subudhi, S Mohanty, R K Joshi, R C Mohanty, S.Nayak (2010) Retention of drug yielding potential of long-term micropopagated gingers through periodic monitoring of genetic stability in vitro. Journal of Pharmacy Research 3(10): 2421-2424. (IF: 1.0).
  • R K Joshi, E Subudhi, B Kar and S Nayak (2010) Comparative genetic analysis of lowland rice cultivars of India using microsatellite markers. Bioresearch Bulletin. 4:213-223.
  • R K Joshi and G J N Rao (2009) Somaclonal variation in submergence tolerant rice cultivars and induced diversity evaluation by PCR markers. International Journal of Genetics and Molecular Biology 1(5):80-88.
  • R K Joshi and T K Bandyopadhyay (2009) Quantitative trait loci (QTL) mapping for crop improvement.  Research Journal of Biotechnology 4(2):67-79. (IF: 0.248)
  • R K Joshi (2008) Tilling-a pathway to crop improvement. Research Journal of Biotechnology 3(4):3-5. (IF: 0.248).
  • R K Joshi and L Behera (2006) Identification and differentiation of indigenous non-Basmati aromatic ricegenotypes of India using microsatellite markers. African Journal of Biotechnology 6(4):348-354. (IF: 0.458).
Forth coming Research Publications
  • R K Joshi, S Mohanty, B Kar and S Nayak (2011) Assessment of genetic diversity in Zingiberaceae through nucleotide binding site (NBS) based motif directed profiling. Biochemical Genetics.
  • B Kar, R K Joshi and S Nayak (2011) Cloning, molecular characterization and transcriptional variability analysis of nucleotide binding site (NBS) type resistance gene candidates from wild Curcuma spp. Molecular Biotechnology.